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Evaluation of Yield Components from Transgenic Soybean Overexpressing Chromatin Architecture-Controlling ATPG8 and ATPG10 Genes
Plant Breeding and Biotechnology 2019;7:34-41
Published online March 1, 2019
© 2019 Korean Society of Breeding Science.

Hyun Suk Cho1, Dong Hee Lee2, Ho Won Jung1, Seon-Woo Oh3, Hye Jeong Kim1*, Young-Soo Chung1*

1Department of Molecular Genetics, College of Natural Resources and Life Science, Dong-A University, Busan 49315, Korea
2Genomine Advanced Biotechnology Research Institute, Genomine Inc., Pohang 37668, Korea
3National Institute of Agricultural Science, Rural Development Administration, Jeonju 54875, Korea
Corresponding author: Young-Soo Chung, chungys@dau.ac.kr, Tel: +82-51-200-7510, Fax: +82-51-200-6536
Hye Jeong Kim, hjkim83@dau.ac.kr, Tel: +82-51-200-5683, Fax: +82-51-200-6536
Received February 18, 2019; Revised February 22, 2019; Accepted February 25, 2019.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
AT-hook proteins are known to co-regulate transcription of genes through the modification of chromatin architecture. In plants, many genes encoding AT-hook proteins have been shown to be associated with increased seed yield or delayed senescence. In this study, we produced transgenic soybean plants overexpressing chromatin architecture-controlling ATPG8 and ATPG10 genes by Agrobacterium-mediated transformation and examined their agronomic traits to identify the yield increase in soybean crop similar to those seen in model plants, Arabidopsis. A total of 16 (3 of pB2GW7.0-ATPG8 and 13 of pCSEN-ATPG10 transformed) transgenic soybean plants were produced and their T1 seeds were harvested. Healthy and well-grown transgenic lines were selected (lines #1 and #2 from pB2GW7.0-ATPG8, and lines #8 and #9 from pCSEN-ATPG10), and the insertion and transcription level of genes were confirmed by PCR and RT-PCR with expected size. Investigation on agricultural traits confirms the increase in yield, plant height, the number of pods, and total seed weight with statistical significance when compared to wild-type soybean plants. The yield component study suggested that overexpression of ATPG8 and ATPG10 genes conferred positive effect on yield in transgenic soybean.
Keywords : Soybean, Chromatin architecture, ATPG8, ATPG10, Agrobacterium-mediated transformation, High-yield


March 2019, 7 (1)
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