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A Gene Functional Study of Rice Using Ac/Ds Insertional Mutant Population
Plant Breed. Biotech. 2018;6:313-320
Published online December 1, 2018
© 2018 Korean Society of Breeding Science.

So-Young Kim1, Chang-Kug Kim2, Min Kang1, Seung-Uk Ji1, Ung-Han Yoon2, Yong-Hwan Kim3, Gang-Seob Lee1*

1Biosafety Division, National Institute of Agricultural Science, Jeonju 54874, Korea
2Genomics Division, National Institute of Agricultural Science, Jeonju 54874, Korea
3Division of Crop Science and Biotechnology, Dankook University, Cheonan 31116, Korea
Correspondence to: Gang-Seob Lee, kangslee@korea.kr, Tel: +82-63-238-4791, Fax: +82-63-238-4704
Received November 6, 2018; Revised November 7, 2018; Accepted November 7, 2018.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Rice is the staple food of more than 50% of the world population. Cultivated rice has the AA genome (diploid, 2n = 24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems havebeen utilized as main insertional mutagens in rice. The Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertional mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been conducted by collaborative works in Korea.
Keywords : Rice, Ac/Ds system, Gene Function


December 2018, 6 (4)
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