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Over- expression of BrTSR53 Gene Improves Tolerance of Rice Plant to Salt Stress
Plant Breeding and Biotechnology 2015;:-
Published online November 30, 2015
© 2015 Korean Society of Breeding Science.

A-Ram Kim, Hyemin Lim, Jung-Il Cho, Chang-Kug Kim, Seung Uk Ji, Soo-Chul Park, Gang-Seob Lee*

Department of Agricultural Biotechnology, National Academy of Agricultural Science, Jeonju, 54874, Korea
Correspondence to: Gang-Seob Lee, kangslee@korea.kr, Tel: +82-63-238-4714, Fax: +82-63-238-4704
These authors contributed equally to this work.
Received November 16, 2015; Accepted November 20, 2015.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Plant is frequently exposed to various abiotic stress. Salt stress is particularly an important abiotic stress that seriously affects plant growth and development. BrTSR53 gene, a putative stress-related gene isolated from Brassica rapa, was used to generate over-expression transgenic rice. The over-expression of BrTSR53 in BrTSR53-OX transgenic rice was confirmed by quantitative RT-PCR and western blot analysis. To elucidate the role of BrTSR53 in stress tolerance, responses of BrTSR53-OX transgenic rice plants to salt stress conditions were examined. BrTSR53-OX #12, #28, and #32 lines were treated with salt stress on MS medium containing 100 mM or 200 mM of NaCl for 5 and 14 days. Morphological analysis revealed differences between the three transgenic BrTSR53-OX rice and the wild-type rice. The germination rates of the three transgenic BrTSR53-OX lines of rice were significantly higher than that of the wild type rice, indicating that they were more tolerant to 200 mM NaCl than the wild type rice. In addition, the three transgenic BrTSR53-OX rice lines had significantly longer length of root and shoot compared to the wild type rice. These results suggest that the BrTSR53 gene played an important role in the tolerance of rice to salt stress. Therefore, it might be a potential target for the purpose of improving salt tolerance of rice and other crops.
Keywords : Brassica rapa, BrTSR53 gene, Salt stress, Transgenic rice