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Development of Improved Ciherang-Sub1 Having Tolerance to Anaerobic Germination Conditions
Plant Breed. Biotech. 2015;:-
Published online June 30, 2015
© 2015 .

Anna Mariel U. Toledo1,2, John Carlos I. Ignacio1, Carlos Casal Jr.1, Zennia Jean Gonzaga1, Merlyn S. Mendioro2, Endang M. Septiningsih1*

1International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines,

2University of the Philippines, Los Banos, Laguna 4031, Philippines

Corresponding author: Endang M. Septiningsih ,, Tel: +63-2-580-5600 , Fax: +63-2-580-5699
Received April 26, 2015; Revised May 6, 2015; Accepted May 6, 2015.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The increased severity and frequency of flooding is causing greater yield reductions in most rice-growing areas. To address this, popular cultivars were improved through introgression of SUB1, an FR13A-derived QTL conferring submergence tolerance at the vegetative stage, using marker-assisted backcrossing (MABC). Ciherang-Sub1, one of these improved near isogenic lines (NILs), showed significantly higher tolerance compared to the original cultivar while retaining its desirable agronomic qualities. However, due to the current shift to direct seeding, seed germination may also be adversely affected by flooding; thus the addition of major QTLs which can confer anaerobic germination (AG) tolerance will be highly beneficial. The AG tolerance QTL, qAG-9-2, also referred to as AG1, derived from Khao Hlan On, a Myanmar landrace, has been introgressed into the elite cultivar IR64 to produce IR64-AG1. This research focused on the transfer of AG1 to Ciherang-Sub1 via MABC, using IR64-AG1, a closely-related donor. Introgression of AG1 and recovery of the Ciherang genome was done in two backcross generations followed by one generation of selfing. The use of a closely-related donor shortened the development period to two years which could have been further reduced if a larger BC1F1 population had been used. Phenotypic evaluation showed that introgression of AG1 significantly increased AG tolerance compared to Ciherang-Sub1, and that the newly developed Ciherang-Sub1+AG1 retained the submergence tolerance from SUB1. The approach is very promising for faster development of improved lines using closely-related cultivars or improved lines as donors for introducing key traits.
Keywords : Rice (Oryza sativa L.), Anaerobic germination (AG) tolerance, Submergence tolerance, Direct seeded, Marker-assisted backcrossing (MABC)

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