Plant Breeding and Biotechnology

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 2287-9366   pISSN 2287-9358

Table. 1.

Table. 1.

TALEN-mediated bar mutations in T0 R6 and R9 plants and their T1 generations.

Mutant lines Generation Mutation types Mutation site (bp) PAT expressionz) TALE-L/Ry) Generation Mutation sites (bp) Amino acids PAT expression TALE-L/R
R6 T0 Start codon deletion/insertion ‒1/‒3 +/‒ ‒/+ T1
(R6,1-40)
+1/‒1/‒2/‒3 Frame shift +/‒ ‒/+


SNP A/G (4) G/T (3), A/G (4), G/T (58), G/A (60)x), G/A (199), G/T (235), A/T (552) Substitution


Stop codon deletion ‒2/‒3/‒4/‒5/‒8 Deletion

R9 T0 Start codon deletion/insertion ‒1 +/+ T1
(R9, 1-31)
‒1/‒3 Frame shift +/‒ +, ‒/+


SNP G/T (3), G/A (22), G/A (57), G/T (137), G/A (167), G/A (219), G/A (412), T/G (550) Substitution


Stop codon deletion ‒1 ‒1/‒4/‒5 Deletion

z)Active (+) and inactive (−) PAT protein expression.

y)Presence (+) and absence (−) of TALE-L and TALE-R constructs.

x)Underlined characters indicate mutations in the TALE:FokI cleavage site.

Plant Breed. Biotech. 2021;9:32-44 https://doi.org/10.9787/PBB.2021.9.1.32
© 2021 Plant Breed. Biotech.