Fig. 1. Schematics of TALE structure and TALE backbone transformation vector for herbicide-resistant bar gene knockout. (A) Simple diagram of TALE targeting of bar gene showing TALE-L and TALE-R with their repeat variable di-residue (RVD) encoding NN, HD, NI, and NG to recognize nucleotides G, C, A, and T, respectively. (B) The TALEN architecture. NLS, nuclear localization signal. (C) Transformation vector construction of pPZP-TaleLR-bar.

Plant Breed. Biotech. 2021;9:32-44 https://doi.org/10.9787/PBB.2021.9.1.32

© 2021 Plant Breed. Biotech.