Plant Breeding and Biotechnology

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Table. 5.

Table. 5.

Common fluorescent dyes for nuclear DNA staining.

Fluorochrome Primary binding mode Wavelength (nm)*

Excitation Emission
Ethidium bromide** Intercalation 530 605
Propidium iodide** Intercalation 540 615
Hoechst 33258 AT-binding 365 465
Hoechst 33342 AT-binding 360 460
DAPI (4,6-diamidino-2-phenylindole dihydrochloride) AT-binding 365 450
DIPI (4’,6-bis (2’-imidazolinyl-4H.5H)-2-phenylindole) AT-binding 365 450
Chromomycin A3 GC-binding 445 570
Mithramycin GC-binding 445 575
Olivomycin GC-binding 440 560

*Dye-DNA complex. **Binds also to double-stranded RNA.

Plant Breed. Biotech. 2021;9:1-19 https://doi.org/10.9787/PBB.2021.9.1.1
© 2021 Plant Breed. Biotech.