Plant Breeding and Biotechnology

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Table. 4.

Table. 4.

Effect of different concentrations of colchicine on morphological charactersitics and chromosome counts in garlic (Allium sativum).

Morphological character Isolate/clone Chromosome count (2n) Size of stomata (relative to diploid control)* Colchicine concentration (w/v)
Dark-green leaves (DG) DG 1 16 S 0.25
DG 2 16 D 0.25
DG 3 16 S 0.25
DG 4 24 D 0.25
DG 5 16 S 0.25
DG 6 16 S 0.25
DG 7 18 D 0.50
DG 8 16 S 0.50
DG 9 Multiple sets I 0.50
DG 10 Multiple sets I 0.50
DG 11 16 S 0.50
DG 12 16 S 0.50
DG 13 16 S 0.50
DG 14 Multiple sets D 0.50
DG 15 Multiple sets I 0.50
DG 16 16 S 0.75
DG 17 16 S 0.75
DG 18 16 S 0.75
DG 19 Multiple sets I 0.75
DG 20 16 S 0.75
DG 21 16 S 0.75
Reduced growth (RG) RG 1 8 D 0.25
RG 2 8 D 0.25
Green and thick leaves (GT) GT 1 32 I 0.50
GT 2 32 I 0.50
GT 3 32 I 0.50
GT 4 32 I 0.50
GT 5 32 I 0.50
GT 6 32 I 0.50
GT 7 32 I 0.50
GT 8 32 I 0.50
GT 9 32 I 0.50
Curled leaves (CL) CL 1 Multiple sets D 0.75
CL 2 Multiple sets D 0.75
CL 3 Multiple sets D 0.75
CL 4 Multiple sets D 0.75

*Adapted from Dixit and Chaudhary (2014): Stomatal measurements were made on the lower epidermis of the second fully-expanded leaf. S: Similar sized stomata to control diploid plant. D: Decreased stomatal size compared to diploid control. I: Increased stomatal size compared to diploid control.

Plant Breed. Biotech. 2021;9:1-19 https://doi.org/10.9787/PBB.2021.9.1.1
© 2021 Plant Breed. Biotech.