Plant Breeding and Biotechnology

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 2287-9366   pISSN 2287-9358

Fig. 4.

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Fig. 4. Detection of indel regions and development of selection markers for BD resistance. (A) PCR amplification of Os01g0601625 and Os01g0601675 regions. (B) Positions of Os01g0601625 and Os01g0601675 regions in the Nipponbare reference genome (IRGSP-1.0) and each indel identified in the Os01g0601625 and Os01g0601675 regions on chromosome 1. The numbers in the upper line indicate the position on chromosome 1 of the Nipponbare reference genome. Gray areas and red boxes in the lower line indicate the predicted gene regions and indel positions, respectively. (C, D) Comparison with Nipponbare (Nip) and Nampyeong (Nam) sequences of Os01g0601625 (C) and Os01g0601675 (D) regions. Arrows indicate the direction and position of primers, and dots indicate the indel regions in each gene. (E, F) PCR band patterns of 1625IND (E) and 1675IND (F) in 27 Korean japonica varieties: 1, Dongjin; 2, DongjinAD; 3, Nampyeong; 4, Hwayeong; 5, Junam; 6, Hopum; 7, Anmi; 8, Ilmi; 9, Hiami; 10, Samgwang; 11, Ungwang; 12, Seolhyangchal; 13, Chilbo; 14, Saenuri; 15, Odae; 16, Joun; 17, Dongan; 18, Geumo; 19, Hwaseong; 20, Nagdong; 21, Giho; 22, Paldal; 23, Jinheung, 24, Jodongji; 25, Damageum; 26, Jungseangeunbangju; 27, Jujido; M, 1 kb Plus DNA ladder.
Plant Breed. Biotech. 2019;7:208-19 https://doi.org/10.9787/PBB.2019.7.3.208
© 2019 Plant Breed. Biotech.