Plant Breeding and Biotechnology

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 2287-9366   pISSN 2287-9358

Fig. 5.

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Fig. 5. Various polymerase chain reaction (PCR) applications using genomic DNA extracted by the TPE method. (A) Genotyping of 12 T2 plants of the rice T-DNA insertional mutant line, PFG_3A-52268. (B) F1 hybridity test using rice microsatellite (RM) marker, RM 11 from 10 F1 plants. (C) Discrimination of SNP type DNA polymorphism for marker-assisted breeding. The A/G SNP located in the promoter region of Gn1a gene which regulates grain number per panicle (Ashikari et al. 2005) was determined from 12 BC1F2 plants of the intermediate breeding line YPF14-582 using the tetra-primer PCR method (Ye et al. 2001).

M: DNA size marker, p1 and p2: parents of F1.

Plant Breeding and Biotechnology 2016;4:99-106
© 2016 Plant Breeding and Biotechnology